Example gallery

Below are some examples that show the functionality of pydna in real-world scenarios.

• Example_Restriction: PCRing a gene out of the genome, and cloning into a vector using restriction and ligation.

• Example_Gibson: Gibson assembly of R. cellulolyticum genomic fragments into a plasmid, from the original Gibson assembly paper doi: 10.1038/nmeth.1318.

• Example_CRISPR: Using CRISPR with homologous recombination to delete genes by making two cuts in the genome, and repair it with an oligo. Used in the industrially relevant K. phaffi.